Maziar Divangahi: Assessment of cell death and proliferation

• Understanding of the mechanisms involved in cell death programs
• Assessment of different cell death modalities
• Assessment of cell proliferation.

Christine McCusker: Immunoglobulins and immune molecules and their use in experimental systems

• What are Antibodies
• How are antibodies made
• Differences between monoclonal, polyclonal and Recombinant antibodies
• Cytokine and chemokines

Jörg Fritz: Flow cytometry

• Cytofluorimetry and FACS - the mechanics
• Analysis of subsets in mixed cell populations
• Assesment of cellular signalling using flow cytometry
• Other applications of flow cytometry

John Presley: Fluorescence & confocal microscopy

• Principles of fluorescence microscopy
• Principles of confocal microscopy
• Confocal vs conventional microscopy
• Applications of confocal microscopy in research

Joaquin Ortega - Electron Microscopy

• Preparation of tissues for light microscopy and electron microscopy
• Different types of microscopy (LM, TEM, SEM, electron probe)
• Theory of electron microscopy
• Practical demonstration of electron microscopy

Carolyn Baglole: Immunocytochemistry, In situ Hybridization & laser capture microdissection

• Molecular basis of hybridization

• Principles of immunocytochemistry

• Applications of in situ hybridization in research

• Combined in situ hybridization/immunocytochemistry
• Laser capture microdissection

Simon Rousseau: Cell culture and cell migration

• Cell culture techniques (sterilization, aseptic methods, media, suspension vs adherent methods, primary vs immortalized cells, preservations)
• Culture contaminations (Bacterial, Fungal, cross-contaminations)
• Assays to study individual steps involved in cell motility (Polarization, Protrusion, Adhesion, Translocation and Retraction)
• Assays investigation motility in 1D, 2D and 3D models.

Stephane Laporte: protein-protein interactions

• Principles of Resonance Energy Transfer (RET): Differences between FRET and BRET
• Probes useful for RET
• Applications of BRET: oligomerization and conformational changes in proteins, and signaling and trafficking of receptors

Reza Farivar: In vivo imaging

• In vivo imaging using MRI