Course Outline

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Andrew Bateman: Spectroscopy

  • Basic Principles
  • UV Spectroscopy - Role in detection and quantitation of biological molecules and as an adjunct in structural characterization
  • Circular dichroism - Basic principles and roles in determining stereochemistry and protein folding
  • Fluorescence - Basic principles and examples
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Maziar Divangahi: Assessment of cell death and proliferation

  • Understanding of the mechanisms involved in cell death programs
  • Assessment of different cell death modalities
  • Assessment of cell proliferation.
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Christine McCusker: Immunoglobulins and immune molecules and their use in experimental systems

  • What are Antibodies
  • How are antibodies made
  • Differences between monoclonal, polyclonal and Recombinant antibodies
  • Cytokine and chemokines
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Jörg Fritz: Flow cytometry

  • Cytofluorimetry and FACS - the mechanics
  • Analysis of subsets in mixed cell populations
  • Assesment of cellular signalling using flow cytometry
  • Other applications of flow cytometry
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John Presley: Fluorescence & confocal microscopy

  • Principles of fluorescence microscopy
  • Principles of confocal microscopy
  • Confocal vs conventional microscopy
  • Applications of confocal microscopy in research
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Joaquin Ortega - Electron Microscopy

  • Preparation of tissues for light microscopy and electron microscopy
  • Different types of microscopy (LM, TEM, SEM, electron probe)
  • Theory of electron microscopy
  • Practical demonstration of electron microscopy
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Carolyn Baglole: Immunocytochemistry, In situ Hybridization & laser capture microdissection

  • Molecular basis of hybridization
  • Principles of immunocytochemistry
  • Applications of in situ hybridization in research
  • Combined in situ hybridization/immunocytochemistry
  • Laser capture microdissection
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Simon Rousseau: Cell culture and cell migration

  • Cell culture techniques (sterilization, aseptic methods, media, suspension vs adherent methods, primary vs immortalized cells, preservations)
  • Culture contaminations (Bacterial, Fungal, cross-contaminations)
  • Assays to study individual steps involved in cell motility (Polarization, Protrusion, Adhesion, Translocation and Retraction)
  • Assays investigation motility in 1D, 2D and 3D models.
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Stephane Laporte: protein-protein interactions

  • Principles of Resonance Energy Transfer (RET): Differences between FRET and BRET
  • Probes useful for RET
  • Applications of BRET: oligomerization and conformational changes in proteins, and signaling and trafficking of receptors
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Reza Farivar: In vivo imaging

  • In vivo imaging using MRI
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