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William Powell - Chromatography
- General principles of chromatography
- Factors which determine resolution & retention times
- Principles of liquid chromatography
- Thin-layer chromatography
- High performance liquid chromatography
- Sample preparation
- Gas chromatography
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Simon Rousseau - Purification of peptides and proteins
- General principles for protein purification
- Protein purification methods
- Size-exclusion chromatography
- Ion exchange Chromatography
- Hydrophobic interaction chromatography
- Chromatofocussing
- Affinity chromatography
- Practical applications
- Overview of protein expression systems
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Andrew Bateman - Mass spectrometry
- General principles of chromatography
- Basic principles - magnetic sector, quadrupole, and time of flight MS
- MS of small molecules – electron impact and chemical ionization MS
- GC-MS - basic principles; single ion monitoring using deuterated standards
- Large molecules - Fast atom bombardment, electrospray, matrix-assisted laser desorption ionization
- Tandem mass spectrometry
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Tommy Nilsson - Proteomics
- Proteomics applications
- Proteomics sample fractionation strategies
- 1-D and 2-D electrophoresis
- Mutidimensional chromatography
- Protease digestion
- Bioinformatic tools for peptide and protein identification
- Quantitation strategies in proteomic analysis
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Christian Rocheleau - Molecular biology
- Analysis of nucleic acids
- Recombinant DNA
- DNA transformation and transfection
- RNAi and anti-sense
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Elizabeth Fixman - Molecular biology
- Site-directed mutagenesis
- Viral vectors
- Protein-protein interactions
- Protein transduction technology
- Cell permeable peptides
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Monzur Murshed - Transgenic & knockout technology
- Transgenic mouse technology
- Knock-out mice
- Applications and pitfalls in the use of transgenic and knockout mice
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Lisbet Haglund - Stem cells
- Clinical applications for stem cell therapeutics
- Sources of stem cells for tissue regeneration
- Propagation/differentiation of stem cells ex vivo
- Pre-clinical methods in cardiovascular repair
- Pre-clinical methods in skeletal regeneration
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Claudia Kleinman - Transcriptomic profiling in human disease
- The basics:
- Composition of the human transcriptome
- Variation of the human transcriptome:
spatial, temporal and technical variation
- Transcriptomic profiling using next-generation sequencing
- RNA-Sequencing technologies
- RNA-Seq Analysis workflow
- Differential gene expression analysis
- Transcriptomic profiling in cancer research - General principles
- Quality control
- DNA-Seq: targeted, exome and whole-genome sequencing
- RNA-Seq
- ChIP-Seq and other applications
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Bernard Turcotte, Christian Rocheleau & Alan Peterson - Laboratory techniques in molecular biology
- Visit to different laboratories for demonstrations of various techniques:
- Microinjections
- Yeast two-hybrid system
- Luciferase assays
- PCR
- Use of databases in molecular biology
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