Course Outline

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William Powell - Chromatography

  • General principles of chromatography
  • Factors which determine resolution & retention times
  • Principles of liquid chromatography
  • Thin-layer chromatography
  • High performance liquid chromatography
  • Sample preparation
  • Gas chromatography
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Simon Rousseau - Purification of peptides and proteins

  • General principles for protein purification
  • Protein purification methods
  • Size-exclusion chromatography
  • Ion exchange Chromatography
  • Hydrophobic interaction chromatography
  • Chromatofocussing
  • Affinity chromatography
  • Practical applications
  • Overview of protein expression systems
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Andrew Bateman - Mass spectrometry

  • General principles of chromatography
  • Basic principles - magnetic sector, quadrupole, and time of flight MS
  • MS of small molecules – electron impact and chemical ionization MS
  • GC-MS - basic principles; single ion monitoring using deuterated standards
  • Large molecules - Fast atom bombardment, electrospray, matrix-assisted laser desorption ionization
  • Tandem mass spectrometry
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Tommy Nilsson - Proteomics

  • Proteomics applications
  • Proteomics sample fractionation strategies
  • 1-D and 2-D electrophoresis
  • Mutidimensional chromatography
  • Protease digestion
  • Bioinformatic tools for peptide and protein identification
  • Quantitation strategies in proteomic analysis
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Christian Rocheleau - Molecular biology

  • Analysis of nucleic acids
  • Recombinant DNA
  • DNA transformation and transfection
  • RNAi and anti-sense
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Elizabeth Fixman - Molecular biology

  • Site-directed mutagenesis
  • Viral vectors
  • Protein-protein interactions
  • Protein transduction technology
  • Cell permeable peptides
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Monzur Murshed - Transgenic & knockout technology

  • Transgenic mouse technology
  • Knock-out mice
  • Applications and pitfalls in the use of transgenic and knockout mice
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Lisbet Haglund - Stem cells

  • Clinical applications for stem cell therapeutics
  • Sources of stem cells for tissue regeneration
  • Propagation/differentiation of stem cells ex vivo
  • Pre-clinical methods in cardiovascular repair
  • Pre-clinical methods in skeletal regeneration
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Claudia Kleinman - Transcriptomic profiling in human disease

  • The basics:
    - Composition of the human transcriptome
    - Variation of the human transcriptome:
       spatial, temporal and technical variation
  • Transcriptomic profiling using next-generation sequencing
    - RNA-Sequencing technologies
    - RNA-Seq Analysis workflow
    - Differential gene expression analysis
  • Transcriptomic profiling in cancer research - General principles
  • Quality control
  • DNA-Seq: targeted, exome and whole-genome sequencing
  • RNA-Seq
  • ChIP-Seq and other applications
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Bernard Turcotte, Christian Rocheleau & Alan Peterson - Laboratory techniques in molecular biology

  • Visit to different laboratories for demonstrations of various techniques:
  • Microinjections
  • Yeast two-hybrid system
  • Luciferase assays
  • PCR
  • Use of databases in molecular biology
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